Coding

Part:BBa_K4447003:Design

Designed by: Rafael Garcia Gomez   Group: iGEM22_TecMonterrey_GDL   (2022-09-28)


RifMo coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1456
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.

Source

The original sequence was reported by Ishikawa et al. (2004). Sequence can be found on GenBank (NG_052045.1).

References

[1]. Ishikawa, J., Yamashita, A., Mikami, Y., Hoshino, Y., Kurita, H., Hotta, K., Shiba, T., & Hattori, M. (2004). The complete genomic sequence of Nocardia farcinica IFM 10152. Proceedings of the National Academy of Sciences of the United States of America, 101(41), 14925–14930. https://doi.org/10.1073/pnas.0406410101